Newsletter March-April 2011  
 
In This Issue

New From Mitegen 
Dual Thickness Micromounts 


Customer Contribution: 
The Story Behind the RT Aligner 


Product Highlights: 
Jena - Phosphotungstate Cluster
 
Reusable Goniometer Bases
 
Magnetic CryoVials
 
LC CryoOil™
 
Crystal Dehydration and Salvage Kit
 
3 Well Crystallization Plates
 

Hot sources: 
Beam time available at CHESS
 

Spotlight: 
Mitegen Technical Tip

Recent Citations

  QUICK LINKS 
 
     
  Shop Mitegen
Now
 
  Upcoming Conferences  
     

Welcome

Thank you for taking the time to review our bimonthly newsletter currently read by over 4000 members of the crystallography and small molecule community. If you know others who may find these updates useful, please be sure to forward this to a friend or colleague.

In each issue in 2011 we will be highlighting 1 or 2 customers’ written case studies. This month, Gregor Blaha of the Department of Molecular Biophysics and Biochemistry at Yale University shares how his need for easier sample preparation led to a new product now available to the industry.

Starting in this issue we will now offer free space for beam line sources to list their available beam times and updates of importance to the industry. Additionally, each month, we will highlight one technical tip or frequently asked question, as well as a few recent papers citing Mitegen products.

Please feel free to contact me directly if you have requests for future newsletter content, any comments, questions or concerns. I’d be glad to hear from you.


Robert Newman
CEO
Mitegen

New from Mitegen
  Dual Thickness MicroMounts™
  • Lowest X-ray Background
  • Greater Rigidity
  • Easier Harvesting
  • Lower Vibration
  • Easier Auto-Centering
  • Maximum Durability
click here for more informationMicroMount DT
Utilizing advanced proprietary micro-fabrication processes, the mounts have a thick, semi-rigid body and a thin, highly X-ray transparent crystal-receiving aperture.This dual thickness maximizes durability and rigidity, while maintaining the ultra-low X-ray background scatter that original MicroMounts™ are known for.

Customer Contribution
The Story Behind the MicroRT™ Aligner
Gregor


 

by Gregor Blaha 
Department of Molecular Biophysics and Biochemistry
Yale University

Last summer, Jimin Wang and I were performing Laue diffraction experiments at the 14-ID beamline (BioCARS facility) at the Advance Photon Source. The data were collected at room temperature in preparation for future time-resolved experiments. Due to significant radiation damage, a fresh protein crystal had to be capillary-mounted after only a few exposures. To collect a complete Laue data set, a large number of protein crystals had to be capillary mounted, which made the MiTeGen MicroRT™ capillary system quite handy. 
Slipping the clear MicroRT™ capillary over the mounted crystal and onto the GB-B3S base without touching the crystal may not sound difficult. However, the rapid transition from working under the microscope for mounting the crystal, to focusing on the base of the pin, to carefully slipping the microRT capillary over the mounted crystal, was quite strenuous to the eyes.  After many hours of capillary mounting, I noticed that it was becoming more difficult to slip the capillary over the crystal without contacting the crystal.  It was at this point of frustration and eye fatigue that I asked myself
 
 
The day after returning from Chicago, I quickly picked up the first phone I laid eyes on and called MiTeGen. Within days, Benjamin Apker of MiTeGen had designed the MicroRT™ Aligner , and, within a month, I had the first prototype in my hands.  After a few minor adjustments, the MicroRT™ Aligner was soon ready for production.
 
About the author:
Gregor Blaha is a postdoctoral associate in the laboratory of T.A. Steitz at Yale University. He and his colleague Christopher Ritacco are currently assisting MiTeGen in the development of other products to further improve the handling of crystals.
 
About time-resolved crystallography:
Time-resolved crystallography permits the observation of macromolecular reactions at the atomic level. For more information see “Laue Crystallography: Time Resolved Studies” by Moffat, K., in “International Tables for X-Ray Crystallography, Volume F: Crystallography of Biological Macromolecules,” edited by M.G. Rossmann and E. Arnold. Ch. 8.2., p. 167-176 (2006).

If you are interested in contributing a Mitegen success story to in a future Newsletter, contact us here
 
  Product Highlights
Phasing Kits - JBS Phosphotungstate Cluster Kit  
 

Phosphotungstate Cluster – makes a world of (electron) difference

Application:

Heavy atom derivatization of biological macromolecules for isomorphous and/or anomalous phasing methods.

Kit Contents: 
6 pre-weighted solid aliquots of Tri-Sodium phosphotungstate cluster at 3.5 mg.

  Tungsten is a strong anomalous scatterer with the L-absorption edges in the energy range of interest for X-ray structure solution. The Tri-Sodium phosphotungstate contains 12 Tungsten centres bridged by oxygen atoms. It has been successfully employed in structural studies because of its high electron-density and very good solubility in aqueous solutions [1].
 


Technical manual:


References [1] Rudenko et al. (2003) 'MAD'ly phasing the extracellular domain of the LDL receptor: a medium-sized protein, large tungsten clusters and multiple non-isomorphous crystals. Acta Cryst. D59:1978.

In North America,

Overseas customers click here to find Jena distributors near you

Reusable Goniometer Bases

Easy swapping of mounts/loops to match your crystal's size and shape, and to optimize harvesting and diffraction outcomes
 
 

Magnetic CryoVials

 
 
 
 
 
 
 
 
 

                LV CryoOil™

Lowest Viscosity of any available perfluoroployether, means:
 
 ✓Easier harvesting

 ✓Less film on your crystal
   
 
   
 

Crystal Dehydration 
and Salvage Kit


Powerful tools for improving the diffraction properties of protein crystals 
 
 

3 Well Crystallization Plate

Easy viewing and retrieval, 
SBS standard
 Mitegen Technical Tip
 Recent Citations of Mitegen Products
Cleaning Mitegen's MicroMounts™, MicroLoops™, MicroMeshes™, MicroGrippers™ and MicroTools™

Manley-King CI, Bergh JJ, Petzer JP (2011) Inhibition of monoamine oxidase by selected C5- and C6-substituted isatin analogues. Bioorganic & Medicinal Chemistry 19: 261

Seidel RW, Goddard R, Hoch C, Breidung J, Oppel IM (2011) On the structure of unsolvated free-base 5,10,15,20-tetra(3-pyridyl)porphyrin. Journal of Molecular Structure 985: 307

Stoner-Ma D, Skinner JM, Schneider DK, Cowan M, Sweet RM, Orville AM (2011) Single-crystal Raman spectroscopy and X-ray crystallography at beamline X26-C of the NSLS. Journal of Synchrotron Radiation 18: 37-40

Tsai M-L, Cronin N, Djordjevic S (2011) The structure of human leucine carboxyl methyltransferase 1 that regulates protein phosphatase PP2A. Acta Crystallographica Section D 67: 14-24

Weng W, Zhang Z, Schlueter JA, Redfern PC, Curtiss LA, Amine K (2011) Improved synthesis of a highly fluorinated boronic ester as dual functional additive for lithium-ion batteries Journal of Power Sources 196: 2171

Zehnder RA, Renn RA, Pippin E, Zeller M, Wheeler KA, Carr JA, Fontaine N, McMullen NC (2011) Network dimensionality and ligand flexibility in lanthanide terephthalate hydrates. Journal of Molecular Structure 985: 109

for a partial list of other citations, click here

Since 2008, Mitegen's tools have been made using the most mechanically robust unoriented polymer available. They are very durable, but because the polymer chains are not oriented (unlike in fibers such as nylon - which are strongly birefringent and produce strong X-ray scatter), the polymer films can be torn if you abuse them during cleaning. Be gentle, and they will last a very long time

The best and easiest way to clean Mitegen's tools is to place them in an ultrasonic bath with a solution containing detergent or a standard laboratory cleaner (e.g., PEX, Alconox.) Hundreds of tools can be cleaned together.

 

If you have deposits that can't be removed ultrasonically, use a Medium (M) or Fine (F) paper wick . Cut back from the widest end using a razor blade so that the diameter fits into a 0.7 mm mechanical pencil. Then dip the other end in water, a detergent solution (e.g., Alconox or PEX) or in isopropanol, and gently stroke the gold-tinted polymer from base to tip. As the wick's tip loses stiffness from use, cut back with a razor blade. In this way you can use a single wick for several cleanings.

 

Wicking MicroMount
 
warning tearing of tip

To dry our tools, place them on absorbent paper and air dry. You can also blow-dry them, but make sure that the tip of the air gun is held at least 2 inches away from the tool tip.

to access other Mitegen Technical Notes, click here