Omscienita products Distributed by Mitegen
|
Memb-PASS™ Membrane Protein Crystallography |
| * Faster * Use Less Protein * Fully Exchange Detergents! |
|
Use less protein and accelerate the process of crystallizing Membrane Proteins using the Memb-PASS™ Screening Kits from Omscientia. Quickly ascertain size and stability with use of the Memb-PASS Differential Filtration Detergent Assay and the Memb-PASS High and Low MWCO Plates in hours using micrograms of protein! Memb-PASS (Prompt Assay of Size and Stability) is a new, patent-pending, protein-efficient method to rapidly screen your integral membrane proteins for proper detergent selection in the preparation for crystallization trials. Also useful for soluble proteins, the Memb-PASS Differential Filtration Detergent Assay allows for a quick and robust assessment of membrane protein stability and rudimentary sizing information in a high-throughput format while quickly helping you select the best detergent complex for your membrane protein. The Memb-PASS Solubility Screen and Memb-PASS Crystallization Screen are specifically designed to work together with the Differential Filtration Detergent Assay and help you significantly reduce the amount of protein, reagents and time spent on the crystallization of Membrane Proteins |
|
Ue less protein and accelerate the process of crystallizing Membrane Proteins using the Memb-PASS™ Screening Kits from Omscientia. Quickly ascertain size and stability with use of the Memb-PASS Differential Filtration Detergent Assay and the Memb-PASS High and Low MWCO Plates in hours using micrograms of protein! Memb-PASS (Prompt Assay of Size and Stability) is a new, patent-pending, protein-efficient method to rapidly screen your integral membrane proteins for proper detergent selection in the preparation for crystallization trials. Also useful for soluble proteins, the Memb-PASS Differential Filtration Detergent Assay allows for a quick and robust assessment of membrane protein stability and rudimentary sizing information in a high-throughput format while quickly helping you select the best detergent complex for your membrane protein. The Memb-PASS Solubility Screen and Memb-PASS Crystallization Screen are specifically designed to work together with the Differential Filtration Detergent Assay and help you significantly reduce the amount of protein, reagents and time spent on the crystallization of Membrane Proteins.
Dr. Wiener, and members of his laboratory at the University of Virginia, have developed, and licensed to Omscientia, the Memb-PASS method for Membrane Protein Crystallography. Memb-PASS starts with a microplate-based detergent screening method that rapidly characterizes the stability and size of membrane proteins exchanged into a large panel of ninety-four carefully selected and unique detergents that feature non-denaturing detergents chosen to suit a wide range of proteins. The DFDA is also useful for protein complexes since if a complex is formed, it would appear larger in the assay. The entire assay utilizes sub-milligram quantities of membrane protein, and requires only several hours to perform!
Additionally licensed to Omscientia is a matched set of pre-crystallization solubility and crystallization screens. After the protein is in a suitable detergent, as determined by the Memb-PASS Differential Filtration Assay, the Memb-PASS Solubility Screen, with twenty-four-conditions, is used to determine the proper protein concentration for crystallization. Once at proper concentration, the protein can then be set up for crystallization with an innovative ninety-six-condition screen, developed and tuned for integral membrane protein crystallization and matched specifically to the Memb-PASS Solubility Screen and Memb-PASS Differential Filtration Assay. The Memb-PASS Crystallization screen is based on conditions that resulted in successful crystallization of membrane proteins. The combined use of these screens can increase the rate of successful crystallization for membrane proteins. Patent Pending
*Rapidly take your membrane protein target through the detergent selection process
*Quickly ascertain suitable solubility conditions for your target (PDC)
*Determine sizing information regarding your PDC in solution avoiding detergent micelle problems
*Use micrograms of protein versus milligrams
* Obtain results of 94 unique, membrane protein specific detergent conditions in a fraction
of the time versus current methods
*Method FULLY exchanges the detergent versus just dilution increasing success in crystallization
*Detergent concentrations tailored specifically for each detergent increasing effectiveness
of detergents and reliability of results
*Memb-PASS system is designed to work together yielding optimal results and eliminating
the guess work of solubility and crystallization screening
*Memb-PASS Crystallization Screen reduces the number of crystallization experiments necessary
to obtain diffraction quality crystals of membrane proteins
References
Vergis JM, Purdy MD, Wiener MC. (2010 Dec 1) A high-throughput differential filtration assay to screen and select detergents for membrane proteins. Anal. Biochem. Dec 2010. 407(1):1-11. Epub 2010 Jul 25.
Memb-PASS Products:
