Newest Crystallization Screen Offerings:
The Pi-minimal Screen was developed at the MRC Laboratory of Molecular Biology (Cambridge, UK) for efficient crystallization screening of soluble proteins. The approach is based on incomplete factorial design.
The Pi-PEG Screen was developed at the MRC Laboratory of Molecular Biology (Cambridge, UK). It has been designed for efficient crystallization screening of integral membrane proteins benefiting from the experience of membrane protein crystallization at the MRC.
JBScreen Classic Kits 1-10 cover 240 (10 kits × 24 conditions each) of the most successful conditions for protein crystallization. Their compositions were obtained by examining the published crystallization conditions of thousands of proteins. JBScreen contains the statistically selected most successful buffers that yielded protein crystals suitable for X-ray diffraction.
Based on the classic sparse matrix crystallization screen first published by Jancarik and Kim in 1991, JBScreen Basic contains 96 unique reagent mixtures for screening a wide range of pH and various salts and precipitants.
Optimized for hydrophobic and membrane proteins.
A highly effective crystallization screen based on data analyses of published kinase structures.
For efficient screening of PEG 3350 and PEG 5000 MME versus 48 different salts.
Systematic screen for pH, anion, cation testing in the presence of polyehtylene glycol.
Optimized sparse matrix screen developed by the Joint Center for Structural Genomics (JCSG).
AA highly effective sparse matrix screen based upon extensive screening of the PDB, with focus on entries by structural genomic initiatives, the BMCD and other protocols.
A systematic crystallization screen based on pentaerythritol polymers as precipitants developed by Ulrike Demmer from the Max-Planck-Institute for Biophysics in Frankfurt
These screens include fluorophore solution and a range of pH � NaCl concentrations that allow you to determine the protein�s thermostability in a given buffer by assessing the protein�s melting profile.