th International Conference on Image Analysis in Three-dimensional Cryo-EM. This significant event is scheduled to take place at Lake Tahoe, California, from March 13-16, 2024. Building on the success of our previous conferences since 2014, which your organization graciously supported and actively participated in, the 2024 Symposium aims to continue the exploration of cutting-edge image analysis approaches in addressing complex biological challenges. The conference will delve into the current state of the art, identify existing limitations, and discuss unresolved issues in the practice of CryoEM, with a particular focus on algorithms and software matters. The Symposium’s tightly focused agenda will encompass diverse topics, such as AI deep learning-based algorithms, data validation, conformational variability, in-situ segmentation, and other emerging subjects in the field of CryoEM. With a history of attracting around 150 participants, a substantial portion of whom are influential methods developers, the event has proven to be a hub for in-depth discussions on algorithmic and methodological advancements.