This Discussion is for established and early-career scientists, post-graduate students and industrial researchers interested in the expanding field of cryo-electron microscopy. Researchers in this area who based in cryo-EM facilities or research institutes, or in departments of biology, chemistry, structural biology, virology, cell biology, molecular biology or biophysics etc are all welcome. Oral and poster presentation opportunities are available to all these groups, and they invite you to submit an oral or poster abstract and join us to make your contribution alongside leaders in the field.