The course will cover the theory, practice, and application of single particle cryo-EM. Participants in the course will learn to perform all steps involved in solving high resolution cryo-EM structures, including sample prep, microscope alignment, data collection, image processing, and model building. Students will have supervised access to CSHL’s Titan Krios and K3 direct electron detector, as well as the Talos L120C. This hands-on course will include lectures by leading experts who will discuss practical and conceptual approaches to structure determination using these techniques, as well as covering a wide range of state-of-the-art applications of cryo-EM in the biological sciences.