The advent of serial crystallography at free electron laser sources and the following adaptation of these methods at 3rd and 4th generation synchrotrons have opened new possibilities to perform room temperature time relolved studies in macromolecular crystallography.
This practical course aims to teach participants know-how of time-resolved serial crystallography and to illustrate the entire pipeline from sample-preparation to a 3D molecular movie. Lectures and practical sessions will be delivered to explain how to prepare micro-crystals of biomolecules, how to use various sample delivery methods for serial crystallography, to design time-resolved experiments, to collect data, and to process serial crystallographic data.