Integrative structural biology is a powerful approach to understand biological macromolecular systems. By combining computational methods with structural science disciplines, spatial and temporal models of macromolecular targets in their in-situ context can be determined. The focus of this workshop is to understand the challenges and opportunities of applying integrative structural biology techniques to one’s research. The fields of light microscopy, mass spectrometry, X-ray crystallography, NMR, cryo-EM, and computational methods will be highlighted. The topics will cover the best practices and current research toward building structural models across different resolution scales. This workshop will also offer hands-on demos to explore what instrumentation is available to be integrated to address conformational changes, flexibility, and dynamics in macromolecular and cellular structures.