BEAMLINE GUIDE
DIAMOND I04


Diamond Light Source
Didcot, Oxfordshire
BART Automounter

I04 is one of seven macromolecular crystallography beamlines at Diamond. The typical working wavelength is 0.9795 Å (12.658 keV) but it is tuneable over the wavelength range 0.69 – 2.066 Å (6 keV – 18 keV).  On request it is also possible to work at a wavelength as low as 0.62 Å (20keV). 1

Features include:

  • Variable focus beam from 10 x 5 to 110 x 100 µm delivering 3 x 3 x 1011  ph/s at the Se K edge
  • Dectris Eiger2 XE 16M detector
  • Smargon multi-axis goniometer
  • High resolution viewing system
  • Accurate sample centering stages
  • Simple beam visualisation procedures
  • Fine resolution attenuation control
  • DLS BART sample changer – 37 Unipucks / 592 samples
  • Vortex fluorescence detector
  • Automated data processing
  • Remote access

Contacts

Ralf Flaig


Tools

We recommend the following tools for their compatibility and ease-of-use.

Recommended Accepted*
Puck Style Uni-Puck
Mount/Loop Style Any

10, 20, 200μm apertures

Rod Length 18mm/SPINE
Base Style B5-R, B-5
*Sample Holders: Cryo-Cooled Samples. Retrieved December 13, 2013

Sample Preparation Kits

Convenient kits of compatible assemblies of MiTeGen recommended products

Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:

  • Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
    • 20 x Dual-Thickness MicroLoops ™ LD  (5 each of 10, 20, 30 and 50 μm aperture sizes)
    • 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
    • 20 x Dual-Thickness MicroLoops ™  (5 each of 300, 400, 500 and 600 μm aperture sizes)
    • 20 x MicroMeshes ™  (5 each of 10, 25, 50 and 25L μm aperture sizes)
  • Installed in 100 B5-R reusable bases or 100 regular bases
  • Packaged and stored in 5 MiTeGen base holders

Uni-puck Starter Kit 2 includes:

  • (7) Universal V-1 Pucks
  • (1) Double Puck Loading Dewar with Lid
  • (1) Puck Wand
  • (1) Puck Dewar Loading Tool
  • (1) Puck Separator Tools
  • (1) Shelved Puck Shipping Cane
  • (1) Bent Cryo-Tong
  • (1) Cryo Express Dry Shipper (CX100)
  • (1) Shipping case for CX100

*custom kits are available, contact MiTeGen with specific requests.


Order Beamline Kits