Glow Discharge System for TEM Grids

The EMS GloQube™ is the first-of-its-kind compact, easy to use, stand-alone glow discharge system.

Key Features Include:

  • Dual independent chambers
  • Hydrophilic/hydrophobic and negative/positive modes
  • Fully automatic, short process times
  • Safe vapor delivery using septum-sealed vials
  • Automatic valving between chambers to prevent cross-contamination
  • Controlled venting to prevent sample disturbance

The primary application of the EMS GloQube™ is the hydrophilization (wetting) of carbon-coated TEM support films and grids which otherwise have the tendency to be hydrophobic. Glow discharge treatment with air will make film surfaces negatively charged and hydrophilic and allow the easy spread of aqueous solutions. This and other processes are outlined below.

Full Description
SKU: M-CEM-EMS-Glo-2$14,000.00
SKU: M-CEM-91003$2,429.00
SKU: M-CEM-96001$280.00
SKU: M-CEM-71175-01$58.00
SKU: M-CEM-71175-02$76.50
Category:

Product Information

Unique Dual Chamber Processing, Safe Handling of Reagents

The GloQube has two independent vacuum chambers: a clean chamber, designed for applications requiring hydrophobic/hydrophilic conversion, typically using air as the process gas; and a vapor chamber, designed for use with reagents such as methanol and alkylamine. With operator safety firmly in mind, reusable septum-sealed reagent vials are used. Loading and removing reagents is convenient and reliable – the vial, located in its holder, is inserted into a shielded needle using a simple bayonet fitting.

To prevent accidental damage, the high voltage lead is shielded. The plasma current is variable by adjustment of the vacuum level using an argon leak valve with the plasma voltage being preset. For maximum sputter coating efficiency, the gas injector system ensures that argon gas enters the chamber close to the plasma discharge. Venting is to argon.

Key Features

  • Dual independent chambers
  • Hydrophilic/hydrophobic and negative/positive modes
  • Fully automatic, short process times
  • Intuitive touch screen control
  • Safe vapor delivery using septum-sealed vials
  • Automatic valving between chambers to prevent cross-contamination
  • Quick and easy sample loading
  • Controlled venting to prevent sample disturbance
  • Consistent, reliable results
  • Three-year warranty

The primary application of the EMS GloQube™ is the hydrophilization (wetting) of carbon-coated TEM support films and grids which otherwise have the tendency to be hydrophobic. Glow discharge treatment with air will make film surfaces negatively charged and hydrophilic and allow the easy spread of aqueous solutions. This and other processes are outlined below.

Glow Discharge Process
Surface State Charge Atmosphere Typical applications
Hydrophilic Negative Air Carbon coated TEM grids
Hydrophilic Positive Air – with magnesium acetate post-treatment Nucleic acid adhesion to carbon films
Hydrophilic Positive Alkylamine Proteins, antibodies and nucleic acids
Hydrophilic Negative Methanol Positively charged protein molecules
(e.g. ferritin, cytochrome c)

Easy sample loading, fast turnaround times

Each chamber can accommodate two 25 x 75 mm glass microscopes slides. Loading could not be easier using draw-style chamber doors and specimen stages. The stages are height adjustable and fitted with removable glass slide holders. For additional convenience – and to allow easy access for chamber cleaning – the stages can be completely removed.

Touch screen control – rapid data input, simple operation

The intuitive touch screen allows multiple users to rapidly input and store preferred process “recipes”. Typical default glow discharge protocols are loaded as standard. Additionally, help files and useful maintenance data such as system on time and time since last clean are readily available to the operator. An Ethernet communications port is included for software updates.

Vacuum, automatic valving and controlled venting

The GloQube™ has automatic valving between chambers which maintains cleanliness by preventing cross-contamination. At the end of a process run, automatic soft venting to atmosphere through filtered inlets ensures TEM grids are not disturbed. The GloQube™ requires a single vacuum pump working in the 0.1 to 1 mbar range. A typical pump time to operational vacuum is 60 seconds.