LEXSY host P10
Glycerol stocks for constitutive expression.
The Leishmania tarentolae laboratory strain P10 is used as host strain for the constitutive LEXSY expression vectors.
For research use only! Not intended for human or animal diagnostic or therapeutic uses.
- Shipping: shipped on dry ice
- Storage Conditions: Store at -80 °C. Avoid freeze/thaw cycles. Upon arrival the glycerol stocks must be stored at -80 °C or inoculated into LEXSY BHI.
- Shelf Life: 12 months
- Manufacturers SKU: LT-101
- MiTeGen SKU: M-LT-101
Product Information
- Content: 3 vials with 1.6 ml each of frozen glycerol stocks of LEXSY host P10 (Leishmania tarentolae laboratory strain P10). These stocks can be stored at -80 °C for at least 1 year. For reactivation see below.
- Organism: Leishmania tarentolae, laboratory strain P10
- Biosafety level: 1, Non-pathogenic for mammalians
- Source: Tarentola annularis
- Reactivation of LEXSY host: Thaw glycerol stock on ice and inoculate the entire content of the vial into 10 ml of LEXSY BHI medium (M-ML-411L). Incubate at 26 °C and dilute as required.
- Preparation of LEXSY BHI growth medium: Dissolve 37 g/l LEXSY BHI powder (M-ML-412L) in deionized water and autoclave for 15 min at 121 °C. Add Hemin and PenStrep. Store at 4 °C and use within two weeks.
- Preparation of glycerol stocks: Add 1.2 ml of growing culture (ca. 6x107 cells/ml) to one vial with 0.4 ml 80 % glycerol, mix, incubate 10 min at RT, 1 h on ice and over night at -20 °C. Transfer to -80 °C. Strains can be stored this way for several years.