LEXSY Plating Kit core
Components for solid medium. Brain-heart infusion- and fetal-calf-serum-based, with NC membranes, spatula, dishes and pipettes.
Core kit for preparation of solidified medium for clonal selection of Leishmania tarentolae. Plasticware to be provides by customer.
For research use only! Not intended for human or animal diagnostic or therapeutic uses.
- Shipping: Shipped at ambient temperature/on gel packs
- Storage Conditions: store components as indicated on individual labels
- Shelf Life: 12 months
- Manufacturers SKU: ML-452
- MiTeGen SKU: M-ML-452
Product Information
Content:
- 2 x 250 ml of Agar base
- 400 ml of buffered liquid medium:
This component is provided in excess to allow for individual batches. - 2 x 50 ml heat-inactivated fetal calf serum (iFCS)
- 2 ml of Hemin stock solution, 500x M-ML-108S:
0,25% solution of Hemin in 30% Triethanolamine, sterile - 5 ml of Pen-Strep stock solution, 200x M-ML-105S:
10.000 units of penicillin (base) and 10.000 μg of streptomycin (base)/ml as penicillin G sodium salt and streptomycin sulfate in 0.85% saline, sterile
All components are provided sterile.
This kit does not contain nitrocellulose membranes, spatula, dishes, pipettes which are provided with the LEXSY Plating Kit comfort (M-ML-451).
Storage conditions:
- Agar base:
Store at room temperature
Stable for 12 months - Buffered liquid medium:
Store at 4 °C
Stable for 12 month - iFCS:
Store < -20 °C
Stable for 12 month - Hemin stock solution, 500x:
Store at 4 °C in the dark
Stable for 12 month - Pen-Strep stock solution, 200x:
Store at -20 °C
Stable for 12 month
Preparation of 500 ml LEXSY plating medium (20 plates, each 25 ml) :
- Microwave-melt 250 ml Agar base in provided glass flask and keep at 55 °C.
- Add pre-warmed (37 °C) buffered liquid medium to ca. 400 ml.
- Add 50 ml iFCS, 1 ml Hemin stock and 2.5 ml Pen-Strep.
- Fill with buffered liquid medium to 500 ml.
- Add appropriate antibiotics (not provided).
- Pour plates with 25 ml pre-warmed (55 °C) serological pipette, avoid air bubbles.
- After solidification, dry the plates open under the laminar flow box for exactly 10 min, than store them closed bottom-up.
- After plating, seal plates with parafilm and incubate bottom- up for 5 - 9 days at 26 °C until colonies appear.
Prepare the plates freshly on the day of plating!