GraVitri 4C Module
The Scientific Need
Preserving Sample Fidelity at the Critical Moment
Eliminating Ambient Vulnerability
In standard manual plunge cooling workflows, delicate biological samples face ambient room temperatures during the critical moments right before vitrification. For sensitive or metastable complexes, this brief thermal exposure causes rapid structural degradation and aggregation, severely compromising downstream high-resolution analysis.
The GraVitri 4°C Humidified Enclosure Module actively eliminates this threat. By maintaining a stable, chilled chamber at 4°C up to the millisecond of the plunge, your sample remains fully protected. Simultaneously, an active humidification system rapidly drives the environment to 90%+ relative humidity in seconds—far outperforming passive sponge methods—to completely halt sample evaporation during the single-sided blotting phase.
Adaptable & Accessible Design
The modular ecosystem introduces operational flexibility to your laboratory bench. This enclosure can be effortlessly installed or completely detached depending on your unique experimental protocols, allowing researchers to precisely calibrate and stabilize active environmental humidification across a customizable temperature range from 4°C to 22°C.
This clever modular architecture also ensures maximum equipment uptime and independence. If the enclosure module ever requires maintenance or repair, it can simply be detached and shipped back to us for swift servicing, while the core plunger station stays in your laboratory for uninterrupted daily use—completely eliminating the costly, scheduled service contracts of over-engineered automated platforms.
Optimized for Single Particle Analysis (SPA)
Protect metastable or transient protein complexes from ambient heat degradation. The GraViTri 4C Module's active 4°C cooling ensures your purified samples remain in their native conformation right up to the moment of vitrification. By minimizing structural heterogeneity and preventing sample dehydration during the blotting phase, you maximize your chances of achieving high-resolution 3D maps.
Components
Additional Information
Flexible Integration for Your Lab
Standalone Module Upgrade
Already own a GraVitri Plunge Cooler? The GraVitri 4C Module is designed for true backward compatibility. Easily upgrade your current setup to include active 4°C environmental control, expanding your lab's capabilities for sensitive biological samples withoutpurchasing an entirely new system.
Complete Prebuilt System
Setting up a new sample prep pipeline? Purchase GraVitri 4C Module bundled with the standard GraVitri Plunge Cooling system. This complete package delivers a fully integrated, cost-effective solution for high-resolution structural biology right out of the box.
Learn About The GraVitri Plunge Cooling System
GraVitri Plunge Cooling System
The GraVitri Plunge Cooling system is built on a time-tested design developed in collaboration with the MRC LMB and Dr. Christopher Russo, this cryo-EM manual plunge cooler has reliably enabled researchers worldwide to vitrify thousands of cryo-EM grids for high-resolution data collection. Available in basic, standard and advanced versions to meet all your sample prep needs.