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Learn Beamline Guides DIAMOND I24

BEAMLINE GUIDE
DIAMOND I24

Diamond Light Source
Didcot, Oxfordshire
BART Automounter

I24 is a tuneable microfocus beamline for MX. It was the first beamline if its kind to be built in Europe and has been operating since 2008. In 2015/2016 the beamline was completely rebuilt with a new dual goniometer endstation and new mirrors. The beamline offers extremely high flux densities with the ability to investigate virus structure, membrane proteins, microcrystals (crystals as small as 1.5 microns have been measured successfully). It combines versatile optics and the most sophisticated detectors available with the most advanced automation systems to enable sample location, data collection and analysis. I24 offers two standard modes of data collection: cryo (SPINE pins) and in situ. Serial (fixed target, thin film and LCP extruder) data collection is also offered. Serial data collection is in a developmental stage and any early experiments can only be done in collaboration with beamline staff1

Contacts

Robin Owen

Other Links

Tools

We recommend the following tools for their compatibility and ease-of-use.

Recommended Accepted*
Puck Style Uni-Puck Uni-Puck
Mount/Loop Style MicroMounts™ & MicroLoops™ LD Any
Rod Length 18mm/SPINE 18mm/SPINE
Base Style B5-Reusable B5-R, B-5
*Robotic Sample Handling, Aina Cohen. Retrieved November 15, 2013.

Sample Preparation Kits

Convenient kits of compatible assemblies of MiTeGen recommended products

Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:

  • Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
    • 20 x Dual-Thickness MicroLoops ™ LD  (5 each of 10, 20, 30 and 50 μm aperture sizes)
    • 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
    • 20 x Dual-Thickness MicroLoops ™  (5 each of 300, 400, 500 and 600 μm aperture sizes)
    • 20 x MicroMeshes ™  (5 each of 10, 25, 50 and 25L μm aperture sizes)
  • Installed in 100 B5-R reusable bases or 100 regular bases
  • Packaged and stored in 5 MiTeGen base holders

Uni-puck Starter Kit 2 includes:

  • (7) Universal V-1 Pucks
  • (1) Double Puck Loading Dewar with Lid
  • (1) Puck Wand
  • (1) Puck Dewar Loading Tool
  • (1) Puck Separator Tools
  • (1) Shelved Puck Shipping Cane
  • (1) Bent Cryo-Tong
  • (1) Cryo Express Dry Shipper (CX100)
  • (1) Shipping case for CX100

*custom kits are available, contact MiTeGen with specific requests.

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