BEAMLINE GUIDE
ESRF ID23-2


European Synchrotron Radiation Facility
Grenoble, France
FlexHCD Automounter

Beamline ID23 is part of the Macromolecular Crystallography Group which supports the study and structure determination of biological macromolecules. The beamline is composed of two independent end-stations. ID23-1 is a fully tunable beamline capable of MAD measurements from 6 to 20 keV energy (2.07 Ã… to 0.62 Ã…). The second station, ID23-2, is fixed energy (14.20 keV, 0.873 Ã…) and is dedicated to perform experiments with very small protein crystals. The optical design of ID23-2 makes use of a horizontally diffracting Si (111) monochromator and Pt coated mirrors in a Kirkpatrick-Baez (KB) geometry as the focusing system.1

Automated Systems at ID23-2:

FlexHCD is a versatile, high performance, sample changer system designed to be used in automatized synchrotron beamlines to handle biological crystallized samples at cryogenic temperatures. It allows the users to automatically transfer samples from the High Capacity Dewar to the diffractometer and back. It is based on a 6-axes robotic arm and a high capacity dewar. FlexHCD has been developed by the EMBL Diffraction Instrumentation Group in collaboration with ESRF.


Contacts

Max Nanao

Shibom Basu


Tools

We recommend the following tools for their compatibility and ease-of-use.

Recommended Accepted*
Puck Style SPINE, Unipuck
Mount/Loop Style Any
Rod Length 18mm/SPINE
Base Style B5-R
*Sample Changer Manual. Retrieved November 18, 2013.

Sample Preparation Kits

Convenient kits of compatible assemblies of MiTeGen recommended products Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:

  • Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:Mitegen B5-R Reusable Base
    • 20 x Dual-Thickness MicroLoops ™ LD  (5 each of 10, 20, 30 and 50 μm aperture sizes)
    • 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
    • 20 x Dual-Thickness MicroLoops ™  (5 each of 300, 400, 500 and 600 μm aperture sizes)
    • 20 x MicroMeshes ™  (5 each of 10, 25, 50 and 25L μm aperture sizes)
  • Installed in 100 B5-R reusable bases or 100 regular bases
  • Packaged and stored in 5 MiTeGen base holders

Uni-puck Starter Kit 2 includes:

  • (7) Universal V-1 Pucks
  • (1) Double Puck Loading Dewar with Lid
  • (1) Puck Wand
  • (1) Puck Dewar Loading Tool
  • (1) Puck Separator Tools
  • (1) Shelved Puck Shipping Cane
  • (1) Bent Cryo-Tong
  • (1) Cryo Express Dry Shipper (CX100)
  • (1) Shipping case for CX100

*custom kits are available, contact MiTeGen with specific requests.


Order Beamline Kits