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Learn Beamline Guides ESRF ID30B

BEAMLINE GUIDE
ESRF ID30B

European Synchrotron Radiation Facility
Grenoble, France
FlexHCD Automounter

ID30B is the second branch of the canted ID30 beam line. This beamline is tunable between 6 and 20 keV (1.9 – 0.62 Å). Two 1.4 m U35 undulators ensure a high intensity (~6 x 1012 phs/s-1/mm2) X-ray beam. The vertical focusing is achieved at different energies by using CRL combinations. The horizontal focusing is performed by an eliptical mirror in the experimental hutch and contains three polished stripes for harmonic rejection at low, medium and high energies. The beam size at the sample position can be changed between 20 and 200 µm in diameter and is cleaned using beam definition apertures. 1

Automated Systems at ID30B:

FlexHCD is a versatile, high performance, sample changer system designed to be used in automatized synchrotron beamlines to handle biological crystallized samples at cryogenic temperatures. It allows the users to automatically transfer samples from the High Capacity Dewar to the diffractometer and back. It is based on a 6-axes robotic arm and a high capacity dewar. FlexHCD has been developed by the EMBL Diffraction Instrumentation Group in collaboration with ESRF. 2

Contacts

Andrew McCarthy

Christoph Mueller-Dieckmann

Other Links

Tools

We recommend the following tools for their compatibility and ease-of-use.

Sample Preparation Kits

Convenient kits of compatible assemblies of MiTeGen recommended products

Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:

  • Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
    • 20 x Dual-Thickness MicroLoops ™ LD  (5 each of 10, 20, 30 and 50 μm aperture sizes)
    • 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
    • 20 x Dual-Thickness MicroLoops ™  (5 each of 300, 400, 500 and 600 μm aperture sizes)
    • 20 x MicroMeshes ™  (5 each of 10, 25, 50 and 25L μm aperture sizes)
  • Installed in 100 B5-R reusable bases or 100 regular bases
  • Packaged and stored in 5 MiTeGen base holders

Uni-puck Starter Kit 2 includes:

  • (7) Universal V-1 Pucks
  • (1) Double Puck Loading Dewar with Lid
  • (1) Puck Wand
  • (1) Puck Dewar Loading Tool
  • (1) Puck Separator Tools
  • (1) Shelved Puck Shipping Cane
  • (1) Bent Cryo-Tong
  • (1) Cryo Express Dry Shipper (CX100)
  • (1) Shipping case for CX100

*custom kits are available, contact MiTeGen with specific requests.

Order Beamline Kits

Recommended Accepted*
Puck Style SPINE, Uni-Puck SPINE, Uni-Puck
Mount/Loop Style MicroMounts™ & MicroLoops™ LD Any
Rod Length 18mm/SPINE 18mm/SPINE
Base Style B5-Reusable B5-R