BEAMLINE GUIDE
ESRF MASSIF-1
European Synchrotron Radiation Facility
Grenoble, France
RoboDiff Automounter
MASSIF-1 is a world leading unique facility for the high throughput, fully automatic characterisation and data collection of macromolecular crystals. The new service is not designed to replace user visits to the synchrotron, but rather to do the hard work of screening crystals or collecting routine data sets through the night, freeing researchers to spend more time on challenging data collection problems and study of the underlying biology.
MASSIF-1 has a highly intense beam (3 × 1012 ph/sec) with a size of 100 μm x 65 μm (H × V FWHM) at a fixed energy of 12.835 keV (0.966 Å). Apertures can be used to define a beam between 100 and 10 μm in diameter. The beamline is optimised for fully automatic data collection and sample evaluation with a new robotic system (RoboDiff) and high capacity dewar (240 spine pins). New automated services use mesh scans to fully evaluate crystals and centre the optimal volume to the beam, this position is then characterised and data collected, or not, depending on user request and parameters defined in the diffraction plan in ISPyB. No restrictions are placed on samples, loop size or crystal quality. As this is a fully automatic high throughput service samples must be registerd in the ISPyB data base with sample names and puck barcodes. 1
Automated Systems at MASSIF-1:
Workflows have been developed that allow the full characterisation and data collection from a large number of crystals with no user intervention. Multiple crystals are selected in MXCuBE which takes care of the mounting / optical centring / unmounting of each sample. MASSIF-1 uses and Passerelle-EDM worflow engine. This workflow first uses a mesh scan and a line scan to centre the best part of the crystal to the X-ray beam.2
Contacts
Matthew Bowler
Other Links
Tools
We recommend the following tools for their compatibility and ease-of-use.
| Recommended | Accepted* | |
|---|---|---|
| Puck Style | SPINE | SPINE |
| Mount/Loop Style | MicroMounts™ & MicroLoops™ LD | Any |
| Rod Length | 18mm/SPINE | 18mm/SPINE |
| Base Style | B5-Reusable | B5-R |
Sample Preparation Kits
Convenient kits of compatible assemblies of MiTeGen recommended products
Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:
- Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
- 20 x Dual-Thickness MicroLoops ™ LD (5 each of 10, 20, 30 and 50 μm aperture sizes)
- 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
- 20 x Dual-Thickness MicroLoops ™ (5 each of 300, 400, 500 and 600 μm aperture sizes)
- 20 x MicroMeshes ™ (5 each of 10, 25, 50 and 25L μm aperture sizes)
- Installed in 100 B5-R reusable bases or 100 regular bases
- Packaged and stored in 5 MiTeGen base holders
Uni-puck Starter Kit 2 includes:
- (7) Universal V-1 Pucks
- (1) Double Puck Loading Dewar with Lid
- (1) Puck Wand
- (1) Puck Dewar Loading Tool
- (1) Puck Separator Tools
- (1) Shelved Puck Shipping Cane
- (1) Bent Cryo-Tong
- (1) Cryo Express Dry Shipper (CX100)
- (1) Shipping case for CX100
*custom kits are available, contact MiTeGen with specific requests.
Order Beamline Kits
-
Small Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 10, 20, 30 and 50 μm aperture DT MicroMounts™) On standard... -
Medium Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 75, 100, 150 and 200 μm aperture DT MicroLoops™) On standard... -
Large Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroLoops™ (25 each of 300, 400, 500 and 600 μm aperture DT MicroLoops™) On standard... -
Universal Puck (Uni-Puck) Starter Kits
To get you up–and–running sending samples to the beamline in easy-to-order bundles. Full Description