DropSaver Lids Mitegen Plates Crystallization

DropSaver Lids for ADE

Custom DropSaver™ Lids for use with ADE systems (Accoustic Drop Ejection)

Reduce evaporative losses
Aid in drop placement
Increase crystal size and quality

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DropSaver™ Lids for ADE – Custom DropSaver™ Lids for use with ADE systems (Acoustic Drop Ejection)
Special high-tolerance DropSaver™ Lids are excellent for protecting the nano-sized drops used in ADE populated experiments.

Developed in collaboration with Dr. Alexei Soares of Brookhaven National Labs

In combination with custom DropSaver™ Lids, ADE devices can be used to populate plates with ultra-small protein droplets, up to 1728 per plate! ^[2]

6 drops per well
(576 drops per plate),
dispensed via ADE

18 drops per well
(1728 drops per plate),
dispensed through slits in a custom DropSaver™ Lid, via ADE2

Reduce evaporation during filling Even with a robot, chances are that your first drops are evaporating measurably before your last drop is set and your plate is sealed. DropSaver™ lids protect your protein drops from ambient air motion that leads to rapid evaporation during the plate loading process
Aid in consistent drop placement Consistent and easy drop setting, especially valuable to when hand displacers are used	as high as 90% reduction in evaporation rates when using DropSaver™ Lids^[1]
Increase crystal size and quality Using lids proved to produce larger and better diffracting crystal for some protiens.[1]

Reduce evaporation during filling
Even with a robot, chances are that your first drops are evaporating measurably before your last drop is set and your plate is sealed. DropSaver™ lids protect your protein drops from ambient air motion that leads to rapid evaporation during the plate loading process

Aid in consistent drop placement
Consistent and easy drop setting, especially valuable to when hand displacers are used

as high as 90% reduction in evaporation rates when using DropSaver™ Lids^[1]

Increase crystal size and quality
Using lids proved to produce larger and better diffracting crystal for some protiens.[1]

Can I just 3D print my own lids?

Yes you can. In fact some prototype and early stage DropSaver™ Lids were made using 3D printing techniques. However these provide to be inferior in regards to uniformity, alignment; and most critically, vapor transmission and evaporation rates. For this reason MiTeGen provides DropSaver™ Lids that are micro-machined in low-permeability molded polystyrene.

DropSaver^TM Lids

Material	Clear Polystyrene (PS)
Thickness	1.4 mm
Length	127.7 mm
Width	85.4 mm

For additional questions about other properties of DropSaver™ Lids please call +1 607 266 877 or email [email protected].

^[1] A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids L. E. Zipper, X. Aristide, D. P. Bishop, I. Joshi, J. Kharzeev, K. B. Patel, B. M. Santiago, K. Joshi, K. Dorsinvil, R. M. Sweet and A. S. Soares, Acta Crystallographica Section F, Volume 70, part 12, Dec. 2014.

^[2] High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density Ella Teplitsky, Karan Joshi, Daniel L. Ericson, Alexander Scalia, Jeffrey D. Mullen, Robert M. Sweet, Alexei S. Soares, Journal of Structural Biology, July 2014, pp 49-58

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DropSaver Lids for ADE

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DropSaver^TM Lids