JBS Solubility Kit
The JBS Solubility Kit is a pre-crystallization screen designed to optimize the composition of the initial protein buffer solution prior to performing crystallization . Since the highly complex properties of proteins are dependent on their environment, buffer solutions play an important role in influencing the solubility and the aggregation behaviour of the protein sample.
Protein aggregation has been shown to inhibit nucleation and crystal growth and to lead to imperfect nuclei and high mosaicity crystals. The JBS Solubility Kit has been developed to examine and optimize the homogeneity and monodispersity of protein samples prior to crystallization trials, through a combination of hanging drop vapour diffusion experiments and dynamic light scattering.
The JBS Solubility Kit contains a set of 24 buffer solutions at different pH-values for setting up hanging drop vapour diffusion experiments to explore the aggregation and precipitation of the protein sample, and a set of 14 additives to be used for further optimization using dynamic light scattering.