Proteinase K – lyophilised
Crystallization grade model protein
For in vitro use only!
- Shipping: shipped at ambient temperature
- Storage Conditions: store at 4
- Shelf Life: 12 months
- Molecular Weight: 28.93 kDa
- CAS#: 39450-01-6
- EC number: 254-457-8
- Form: lyophilised powder
- Manufacturers SKU: CO-404
- MiTeGen SKU: M-CO-404
Proteinase K is a fungal protease from Tritirachium album. It belongs to the subtilisin family with the classic catalytic triad of Asp39-His69-Ser224 at its active site. The enzyme is very stable over a wide pH-range, at various salt concentrations and is even active in SDS concentrations up to 0.5%.
Source: Tritirachium album
Warm the Proteinase K powder to room temperature before opening.
We recommend to resuspend the enzyme in 50 mM HEPES pH 7.0 to a final concentration of 10-20 mg/ml.
Crystals can be grown using the sitting drop or hanging drop vapor diffusion method.
Following crystallization conditions are suggested for Proteinase K crystal growth[2,3]:
- 1.2 M Ammonium Sulfate, 0.1 M Tris-HCl pH 8.0
- 1.0 M NaNO3, 100 mM Citrate Buffer pH 6.5
Proteinase K can be utilized as model protein in crystallization experiments and X-ray structure analysis.
 Larson et al. (2009) High-resolution structure of proteinase K cocrystallized with digalacturonic acid. Acta Cryst. F 65:192.
 Müller-Diekmann (2007) On the routine use of soft X-rays in macromolecular crystallography. Part IV. Efficient determination of anomalous substructures in biomacromolecules using longer X-ray wavelengths. Acta Cryst. D 63:366.
 Wang et al. (2006) What can be done with a good crystal and an accurate beamline? Acta Cryst. D 62:1475.