BEAMLINE GUIDE
Australian Synchrotron MX 2
Australian Synchrotron
Clayton, Victoria
SAM (SSRL) Automounter
The MX beamlines are general purpose crystallography beamlines dedicated to chemical and biological structure determination. Single crystals can be analysed using single (SAD) or multiple wavelength anomalous dispersion (MAD). Automated energy changes are available (energy range for MX2 is 8kEv – 15.5 kEv), and samples can be mounted using a SSRL robot. Expert technical assistance is available to help users who are not specialist crystallographers. The MX2 beamline is a finely focused in-vacuum undulator beamline and is equipped with a microcollimator. It is ideal for weakly-diffracting, hard-to-crystallise proteins, viruses, protein assemblies and nucleic acids as well as smaller molecules such as inorganic catalysts and organic drug molecules.1
Automated Systems at MX2:
This beamline is equipped with a SAM (SSRL Automated Mounting) system.
Specifications of MX2 are:
- Energy Range (User Controlled) (keV) – 8-21
- Beam size at sample position (FWHM HxV) (µm) – 25 x 15
- Camera zoom – zoom camera 8X
- Minimum recommended crystal size – protein crystals -> 10 µm; chemical crystals as small as 5 µm (with strongly scattering elements)
- Detector – Dectris Eiger 16M
- Additional features –
- Micro-collimator
Contacts
Dr. Rachel Williamson
Dr. Alan Riboldi-Tunnicliffe
Other Links
Tools
We recommend the following tools for their compatibility and ease-of-use.
| Recommended | Accepted* | |
|---|---|---|
| Puck Style | Uni-Puck | Uni-Puck |
| Mount/Loop Style | MicroMounts™ & MicroLoops™ LD | Any |
| Rod Length | 18mm/SPINE | Any |
| Base Style | B3-Reusable, B3 | B3-Reusable, B3 |
| *MX-Beamlines Retrieved September 13, 2017. | ||
Sample Preparation Kits
Convenient kits of compatible assemblies of MiTeGen recommended products
Beamline Sample Preparation Kits are available in 3 aperture size assortments based on your crystal sizes and include:
- Assemblies of 100 Crystal Mounts, on 18 mm pins, available in the following assortment:
- 20 x Dual-Thickness MicroLoops ™ LD (5 each of 10, 20, 30 and 50 μm aperture sizes)
- 40 x Dual-Thickness MicroMount ™ (10 each of 75, 100, 150 and 200 μm aperture sizes)
- 20 x Dual-Thickness MicroLoops ™ (5 each of 300, 400, 500 and 600 μm aperture sizes)
- 20 x MicroMeshes ™ (5 each of 10, 25, 50 and 25L μm aperture sizes)
- Installed in 100 B5-R reusable bases or 100 regular bases
- Packaged and stored in 5 MiTeGen base holders
Uni-puck Starter Kit 2 includes:
- (7) Universal V-1 Pucks
- (1) Double Puck Loading Dewar with Lid
- (1) Puck Wand
- (1) Puck Dewar Loading Tool
- (1) Puck Separator Tools
- (1) Shelved Puck Shipping Cane
- (1) Bent Cryo-Tong
- (1) Cryo Express Dry Shipper (CX100)
- (1) Shipping case for CX100
*custom kits are available, contact MiTeGen with specific requests.
Order Beamline Kits
-
Small Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 10, 20, 30 and 50 μm aperture DT MicroMounts™) On standard... -
Medium Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroMounts™ (25 each of 75, 100, 150 and 200 μm aperture DT MicroLoops™) On standard... -
Large Aperture Assortment Beamline Sample Preparation Kit
100 Dual Thickness MicroLoops™ (25 each of 300, 400, 500 and 600 μm aperture DT MicroLoops™) On standard... -
Universal Puck (Uni-Puck) Starter Kits
To get you up–and–running sending samples to the beamline in easy-to-order bundles. Full Description