In Situ-1 Crystallization Plate

1. Thin COC Film A thin COC film gives you excellent transparency for better diffraction data when using either X–ray or UV light. Compare the following to see the difference:

2. Micro–channels Allow vapor evaporation between reservoir and protein wells Strongly inhibits fluid transfer between protein and reservoir wells Plates can be rotated to any orientation without liquid transfer out of the reservoir

3. Micro–ledges The reservoir wells contain special ledges that: Force fluid into the reservoir, ensuring uniform well–filling for larger reservoir volumes in a low profile plate Keep fluid away from your top sealing film More fluid in a shorter reservoir Inhibits transfer out of the reservoir well during rough handling and tipping

Ship–able

The dual–action of the micro–channels and micro–ledges makes In Situ–1 plates capable of being shipped long distance with no cross–contamination of fluid from the reservoirs to the protein growth areas.

Populated plates shipped via Fedex from Ithaca, New York to Adelaide, Australia showed no cross–contamination of fluid from the reservoirs to the protein growth areas

Versatility

The inhibition of cross–contamination between the reservoirs allows In Situ–1 plates to be used with the sitting drop or hanging drop method of protein crystallization.

Download the information sheet from below:

In-Situ-01™ Crystallization Plate Info

For additional questions please call +1 607 266 877 or email support@mitegen.com.

Tech Drawings

Viewing Angles #1

Viewing Angles #2

Looking for Robot drop setting files or plate schematics? Contact us here!

Want to use the Situ-1 Crystallization Plates with the Mosquito robot?

You may wish to take a look at the following paper:

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source

You can use Ctrl+F to search for 'In Situ-1' to see all the instances in which they describe and instruct usage of the In Situ-1 Plates with the Mosquito Robot.

Guide yourself to Figure 2, which shows images of how the Mosquito interface appears in each step of setting up use with the In Situ-1 crystallization plates. You can follow the instructions located in Figure 2 to complete the process.

If you're still struggling to set up the Mosquito with the In Situ-1 Plates, or if you have other questions that require assistance, please let us know. Use the contact form to the right.