JBS Halo Kits

The search for suitable heavy-atom derivatives can be quite tedious. Binding of heavy atoms often disrupts the crystal lattice. Halogenated ATP and GTP analogs provide an alternative method that allows rational incorporation of heavy atoms into a large number of physiologically relevant enzymes

SKU: M-PK-101$605.00
SKU: M-PK-102$385.00
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Product Information

The search for suitable heavy-atom derivatives can be quite tedious. Binding of heavy atoms often disrupts the crystal lattice. Halogenated ATP and GTP analogs provide an alternative method that allows rational incorporation of heavy atoms into a large number of physiologically relevant enzymes:

  • In the human genome alone, estimated 5,000 to 10,000 proteins interact with ATP or GTP, e.g. protein and nucleotide kinases, motor proteins, chaperones or the superfamily of GTPases, respectively.
  • The incorporation of iodine or bromine allows MIR or MAD phasing for proteins with molecular weights of at least up to 50 kDa. Importantly, for MIR experiments, such derivatives are likely to be isomorphous to the native crystals
  • The binding kinetics of 2′-halogenated ATP analogs to most enzymes so far investigated (including myosin, creatine kinase, hexokinase, pyruvate kinase and UMP/CMP kinase) are similar to those of non-substituted ATP.
  • The complexes of 2′-halogenated GTP analogs with the GTPases p21ras or Rab5 have dissociation constants well suitable for crystallization.
  • Several proteins including kinesin, non-claret disjunctional protein and TMP kinase have been successfully crystallized with 2′-halogenated analogs.

The JBS Halo-ATP Kit contains 8 halogenated nucleotide analogs as lyophilised sodium salt:

  • 2′-Iodo-dADP (#NU-109)
  • 2′-Iodo-dATP (#NU-101)
  • 2′-Bromo-dADP (#NU-110)
  • 2′-Bromo-dATP (#NU-102)
  • 8-Iodo-ADP (#NU-111)
  • 8-Iodo-ATP (#NU-113)
  • 8-Bromo-ADP (#NU-112)
  • 8-Bromo-ATP (#NU-114)

The JBS Halo-GTP Kit contains 4 halogenated nucleotide analogs as lyophilised sodium salt.

  • 8-Iodo-GDP (#NU-123)
  • 8-Iodo-GTP (#NU-125)
  • 8-Bromo-GDP (#NU-124)
  • 8-Bromo-GTP (#NU-118)

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Applications:
Incorporation of halogenated GTP analogs into nucleotide binding enzymes for isomorphous/ anomalous phasing.