JBScreen Detergents

The JBScreen Detergents can be used throughout the protein purification process or can be added afterwards by dialysis or ion-exchange chromatography (detergent exchange). Detergent exchange can be vital for obtaining well-diffracting membrane-protein crystals [1].

Full Description

 

$199.00

SKU: M-CS-521 $199.00

$199.00

SKU: M-CS-522 $199.00

$199.00

SKU: M-CS-523 $199.00

$199.00

SKU: M-CS-524 $199.00

$599.00

SKU: M-CS-525 $599.00

$1,699.00

SKU: M-CS-308 $1,699.00

Product Information

The JBScreen Detergents contain 4x 24 unique detergents that are compatible with most common crystallization reagents and are therefore perfectly suited for membrane protein solubilization:

  • Ionic detergents
  • Non-ionic detergents
  • Zwitterionic detergents
  • Non-detergent Sulfobetaines
  • Synthetic Lipids

The JBScreen Detergentscan be used throughout the protein purification process or can be added afterwards by dialysis or ion-exchange chromatography (detergent exchange). Detergent exchange can be vital for obtaining well-diffracting membrane-protein crystals [1].

JBScreen Detergents are also valuable for additive screening with detergents and detergent mixtures [2,3] in combination with the JBScreen Membrane. This combination will enable you to screen a broad range of crystallization conditions, while concentrating on the most successful detergents – and therefore making crystallization screening of membrane proteins much more efficient and less time consuming.

References:
[1] Rosenow et al. (2003) The influence of detergents and amphiphiles on the solubility of the light harvesting complex. Acta Cryst. D59:1422
[2] Adir (1999) Crystallization of the oxygen-evolving reaction centre of photosystem II in nine different detergent mixtures. Acta Cryst. D55:891
[3] Koronakis et al. (2000) Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature 405:914