MicroMeshes™ are ideal for very small (<30 μm) samples, allowing them to be sieved out of solution. They also provide continuous and delicate support for, e.g., thin plates, rods and tissue samples.
MicroMeshes are the tool of choice for microcrystal crystallography and diffraction experiments, especially at microfocus beamlines. They have been used in de novo protein structure determination from crystals as small as 5 µm. Multiple small samples can be retrieved and mounted together, spread out over the mesh. The diffraction properties of each sample can be separately interrogated using a small (<100 μm) X-ray beam. Dip into your drop and shoot to evaluate the results of early crystallization trials.
The polymer tips are attached to beveled non-magnetic 0.025" (0.64 mm) diameter stainless steel rods. Compatible with all standard X-ray hardware, they can be inserted into 0.7 mm mechanical pencils or micromanipulators for easy handling.
Standard MicroMeshes™ – mesh filled apertures with diameters of 400 or 700 μm and mesh openings of 10, 25 and 50 μm.
MicroMeshes SH™ – 80 μm diameter mesh area with 15 μm openings. The small head fits entirely within the X-ray beam diameter typical of standard synchrotron beam lines and of focused laboratory sources. You don't have to see your tiny sample to align it: just center the 80 μm head in the beam, and your sample will be within it. The smaller head also makes it easier to mount a single sample, and produces less background scatter when the beam is aligned along the plane of the film.
Indexed MicroMeshes™ - 300 or 400 μm mesh areas with 25 μm openings. Index marks and numbers make it easier to locate (and then to relocate) a given sample. Identify the most promising samples on your home microscope, and then easily find them again, e.g., at a microfocus X-ray source. Additional diagonal tabs allow the front/back orientation of the mesh and a crystal's position on the mesh to be uniquely determined in only a 100 μm field of view. Crystals can sometimes be more clearly seen in the larger square openings of the 400/25-IN2 design. The flat top of the 300/25-IN1 design makes it easier to scrape/scoop samples off of the bottom of a well or slide.
Click here to see close-ups of the mesh tips.Features
- Sieving action allows easy retrieval and mounting of small (<30 μm) samples.
- Continuous support for fragile samples.
- Rigid yet thin construction. Minimal bending in viscous solutions and in cold gas streams.
- Lowest background X-ray scatter of any commercial mount.
- Excellent tip visibility at large inclination angles (important when retrieving samples from small wells) due to beveling of the supporting rod. Rods can be cut using cutters or bent using pliers.
- Accurate and reproducible sample positioning in the X-ray beam.