Room temperature screening is especially important in protein crystallography. Roughly 98% of low-temperature data sets collected on structural biology beam lines do not yield diffraction data of sufficient quality to determine a structure. Room temperature measurements are essential to determine whether the cause of poor diffraction is poor as-grown crystal quality, sample damage caused by ligand and/or cryoprotectant soaks, and/or sample damage caused by the flash cooling process itself. Far too much effort is wasted searching for cryoprotection conditions for crystals that are poorly ordered to start with. Far too much synchrotron beam time is wasted on crystals that should have been rejected in home source screening.
The MicroRT™ system provides an extremely cost effective tool for increasing the efficiency of your diffraction pipeline.